1) The PLgel series is an efficient separation column launched by Agilent. It requires a small number of connections, saves solvents, and has good separation effect, but is expensive. This series of molecular weights is suitable for medium and low polar solvents such as tetrahydrofuran, chloroform, dichloromethane, etc. Although it can also be used in DMF, the separation effect is poor.

2) Polargel is both a high-efficiency separation column, and is more suitable for high-polar solvents such as DMF, DMSO, and water. We have compared the effects of PLgel and Polargel and waters separation columns in DMF. Polargel is far ahead in accuracy and reliability. After verifying the effect of single root detection and taking into account its expensive price, we only equipped one column (the series has only two columns, the other is an ultra-high molecular weight interval).

To facilitate the writing of the paper, the following detection conditions description can be directly copied.

THF phase Waters model: GPC test was performed in THF solution at 35 °C with an election rate of 1.0 mL min−1 on a Waters-E2695 GPC system equipped with a refraactive index detector. The apparent molecular weights were determined on a PLgel Mixed-B and a PLgel Mixed-C columns using linear poly(methyl methacrylate) or PS (polystyrene) as standards.

THF phase Agilent model: GPC test was performed in THF solution at 35 °C with an elastic rate of 1.0 mL min−1 on a Agilent Technologies 1260 Infinity II GPC system equipped with a refraactive index detector. The apparent molecular weights were determined on a PLgel Mixed-B and a PLgel Mixed-C columns using linear poly(methyl methacrylate) or PS (polystyrene) as standards.

DMF phase Agilent model: GPC test was performed in DMF solution at 50 °C with an elastic rate of 1.0 mL min−1 on a Agilent Technologies 1260 Infinity II GPC system equipped with a refraactive index detector. The apparent molecular weights were determined on a single Polargel-M column using linear poly(methyl methacrylate) or PS (polystyrene) as standards.

Water phase Waters model: GPC test was performed in H2O solution at 40 °C with an electrification rate of 1.0 mL min−1 on a Waters-E2695 GPC system equipped with a refraactive index detector. The apparent molecular weights were determined on a single PL aquagel-OH MIXED-M column using linear PEG as standards.